Eicosapentaenoic acid is improved to a high produced a Yarrowia lipolytica (Yarrowia lipolytica) optimal strains

摘要

The ω-3 polyvalent eicosapentaenoic acid is an unsaturated fatty acid ["EPA"] includes more than 50% by weight, measured as weight percent of total fatty acids ["% TFA"] and the EPA% TFA,% TFA The ratio of the measured linoleic acid, and describes the modified strain of at least 3.1 and a oil can produce oily yeast Yarrowia lipolytica (Yarrowia lipolytica) as. These strains, other heterologous Δ9 elongase, Δ8 desaturase, Δ5 desaturase, Δ17 desaturase, in addition to the Δ12 _{desaturase, C 16/18} elongase, at least one of the Δ9 elongase / Δ8 desaturase multi partzyme overexpressed, further diacylglycerol choline phosphotransferase, it is also possible to overexpress the malonyl-CoA synthetase and / or acyl-CoA lysophospholipid acyltransferases. Expression of at least one of the peroxisome biogenesis factor protein is down-regulated. Method of producing EPA in the host cells, the oil obtained from the cell, and the products derived therefrom is claimed.