ANTI-MORPHINE ANTIBODY, METHOD FOR MEASURING MORPHINE USING THE SAME, AND MORPHINE MEASURING KIT CONTAINING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a superior anti-morphine antibody capable of recognizing morphine specifically, and having low recognition ability to heroin, codeine, cocaine and ketamine, and to thereby facilitate measurement of morphine in a sample.SOLUTION: This anti-morphine antibody to be prepared is selected from a group comprising a single-chain antibody (scFv), a Fab fragment, a F(ab')fragment, a Fv fragment and a perfect antibody, which comprise anti-morphine antibody molecules capable of recognizing morphine specifically, having low recognition ability to heroin, codeine, cocaine and ketamine, and containing a heavy chain variable region and a light chain variable region, and further the anti-morphine antibody has, in the heavy chain variable region, (a) an amino acid sequence described on the 31th place to the 35th place of SEQ ID NO.1 in the sequence table as CDR-H1, (b) an amino acid sequence described on the 50th place to the 65th place of SEQ ID NO.1 as CDR-H2, and (c) an amino acid sequence described on the 98th place to the 110th place of SEQ ID NO.1 as CDR-H3, and/or also has, in the light chain variable region, (a) an amino acid sequence described on the 160th place to the 174th place of SEQ ID NO.1 in the sequence table as CDR-L1, (b) an amino acid sequence described on the 190th place to the 196th place of SEQ ID NO.1 as CDR-L2, and (c) an amino acid sequence described on the 229th place to the 237th place of SEQ ID NO.1 as CDR-L3.