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Positively charged species as a binding reagent in the separation of protein aggregates from monomers
Positively charged species as a binding reagent in the separation of protein aggregates from monomers
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机译:带正电荷的物质作为结合剂,可从单体中分离蛋白质聚集体
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摘要
The invention provides methods for detecting the presence of an aggregate in a sample by contacting the sample suspected of containing an aggregate with an aggregate-specific binding reagent under conditions that allow the binding of the reagent to the aggregate, if present; and detecting the presence of the aggregate, if any, in the sample by its binding to the reagent; where the aggregate-specific binding reagent typically has a net charge of at least about positive one at the pH at which the sample is contacted with the ASB reagent, is attached to a solid support at a charge density of at least about 60 nmol net charge per square meter, and binds preferentially with aggregates over monomers when attached to the solid support. Methods for detecting the presence of oligomer are also provided. Compositions for use in the methods are provided.
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