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Coat it does the freezing preservation method of threely-dimensional laying out the cell of multiple types and the base of the depression

机译:外套进行三维保存的冷冻保存方法,对多种类型的细胞和凹陷的底部进行布局

摘要

PROBLEM TO BE SOLVED: To provide a method for freeze-preserving cells enabling freeze/thaw trouble to be suppressed to a minimum through reducing both labor and time for complicated cell culture operation, and to provide a method for restoring a cell-culturing condition by rapidly recovering various cell functions including cell proliferativeness after thawing.;SOLUTION: The method for freeze-preserving cells comprises freezing the cells in a suspended state in a cell culture apparatus using a freeze-preserving liquid. A method for culturing cells comprising the following process: A warmed culture solution is added to cells freeze-preserved by the method in the cell culture apparatus to rapidly thaw the cells to restore the cells with their functions kept intact.;COPYRIGHT: (C)2008,JPO&INPIT
机译:解决的问题:提供一种冷冻保存细胞的方法,该方法通过减少复杂细胞培养操作的劳力和时间将冷冻/解冻问题抑制到最小,并提供一种通过以下方法恢复细胞培养条件的方法:解冻后迅速恢复各种细胞功能,包括细胞增殖。解决方案:冷冻保存细胞的方法包括使用冷冻保存液将细胞以悬浮状态冷冻在细胞培养装置中。一种细胞的培养方法,包括以下步骤:将温热的培养液加入到通过该方法在细胞培养装置中冷冻保存的细胞中,以快速融化细胞以恢复其功能完整的细胞。版权所有:(C) 2008,日本特许厅

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