PCR primer set, PCR reaction solution, detection method for food poisoning bacteria

摘要

It specifically amplifies the nucleic acid of two or more food poisoning bacteria among Cereus, Campylobacter, Escherichia coli, Listeria, Salmonella, Staphylococcus aureus and Vibrio parahaemolyticus. Thereby, these food poisoning bacteria can be specifically detected simultaneously. SEQ ID NO: 1 and 2, SEQ ID NO: 3 and 4 primer set for detecting Cereus, SEQ ID NO: 5 and 6 primer set for detecting Campylobacter, SEQ ID NO: 7 and 8 primer set for detecting E. coli, Listeria detecting sequence A primer set of SEQ ID NOs: 11 and 12 for detecting Salmonella, a primer set of SEQ ID NOs: 13 and 14 for detecting S. aureus, a primer set of SEQ ID NOs: 15 and 16 for detecting Vibrio parahaemolyticus Among them, nucleic acids of food poisoning bacteria are amplified by PCR using a primer set for PCR consisting of two or more primer sets.