Cell production method, cell, modified PC12 cell, gene, and mutant phosphoinositide-3 kinase

摘要

It is an object of the present invention to provide a technique capable of providing a modified PC12 cell having a new characteristic and, at the same time, creating a new cell having the characteristic possessed by the modified PC12 cell. Modified PC12 cells having the following properties (a) and (b) are provided. (A) Nerve differentiation is not induced by the action of a nerve growth factor, (b) Mutant phosphoinositide-3 kinase that does not activate Akt (serine / threonine kinase) by the action of a nerve growth factor is expressed. A method for producing a cell that produces a mutant cell that expresses a mutant phosphoinositide-3 kinase that does not activate Akt (serine / threonine kinase), a cell obtained by the method for producing the cell, a gene encoding the mutant phosphoinositide-3 kinase, And a mutant phosphoinositide-3 kinase encoded by the gene is also provided.