Analysis sorting apparatus of the cell analysis and sorting how cell adhesion light control substrate, the cell

摘要

The analyzed alive cells were fractionated, in order to culture, more conveniently, it can work in real time, allowing cultivation while to remove and purify unwanted cells from the cultured cells. Furthermore, analyzes of sorted cells from cultured cells desired, to increase the purity of the cells, recovery, and viability. The use of cell-adhesive optical control substrate formed by depositing a transparent substrate cell adhesive light control material bound cell adhesive material through a photolabile group in a cell non-adhesive material. The detection cell image, analyze and obtain the location information of the desired cells. Based on this information, it disconnects the cell adhesive material and light control cell / cell between the light irradiation of the second. On the other hand, by changing the cell non-adhesive cell adhesion from the surface of the substrate by light irradiation of the first, causing the detachment of the cell / base material between. It can be analyzed alive cells is thereby separated.