MARINE BACTERIAL LIQUID FOR DEGRADATION OF HIGH-MOLECULAR-WEIGHT POLYCYCLIC AROMATIC HYDROCARBONS AND THE PREPARATION METHOD THEREOF

摘要

A composite marine bacterial liquid for the synergic degradation of high-molecular-weight polycyclic aromatic hydrocarbons (HMW PAHs) and the preparation method thereof. The composite marine bacterial liquid is generated by mixing the fermented culture liquid of a stain of Cycloclasticus sp. PY97M and a stain of Marinobacter sp. D15-8W, the cell concentration of each is 10⁷-10⁹ CFU/mL. The preparation method includes: inoculating the seed liquid of Cycloclasticus strains and Marinobacter strains individually into the sodium acetate medium or M8 medium, fermenting under the condition of defoaming by flowing addition of defoamer, and mixing the fermented cultures to obtain the composite marine bacterial liquid, then preparing a 5-fold concentrated liquid by centrifuging and storing at 4° C.