首页>
外国专利>
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
展开▼
机译:含有非天然氨基酸苯基硒代半胱氨酸的蛋白质的基因编程表达
展开▼
页面导航
摘要
著录项
相似文献
摘要
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel sythetases molecules, methods for identifying and making the novel synthetases, methods for producing containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lapidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.
展开▼