Synthesis of Threefold Glycosylated Proteins using Click Chemistry and Genetically Encoded Unnatural Amino Acids

摘要

Eukaryotic proteins and in particular cell-surface proteins are frequently glycosylated,[1–2] which has fueled the interest of chemist to develop new methods for the synthesis of glycosylated proteins.[3–15] Protein glycosylation is essential for the proper function of the respective proteins and in the case of glycosylated protein therapeutics, such as erythropoietin, the activity of the protein strongly depends on glycosylation patterns.