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Methods for producing soluble membrane-spanning proteins
Methods for producing soluble membrane-spanning proteins
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机译:产生可溶性跨膜蛋白的方法
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摘要
Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.
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