Tritium-labeled hyaluronic acid, method of labeling hyaluronic acid by tritium and use thereof

摘要

The present invention relates to a kind of tritium-labeled hyaluronic acid and preparation methods. Where preparation is oxidized to aldehyde and alcohol based on redox strategy and is restored back to the position parts polysaccharide 6-glukosamine. The result is that in the range of the molecular weight of natural derivative is 800 to 1 000 000 gram. Mole-1 shows high stability and specific activity, these reducing agents used by specific activity, only. Method of the invention quickly, inexpensively, generates minimal amount of radioactive waste and forms ballast. The hyaluronic acid so marked has made the bio distribution tested, and is studying where its stability is verified in brown rat, by detection urinary HA after intravenously administrable wherein. The tritium-labeled hyaluronic acid according to the present invention can be used in biomedicinal application.