Method for the preparation of packaging cells comprising sequences encoding adenovirus e1a and e1b, recombinant molecules used in the method and cells comprising the molecules

摘要

In the absence of substantial sequence overlap between a recombinant adenoviral vector and the genome of a packaging cell, helper-dependent E1-containing particles (HDEP) can be formed at low frequency. The invention provides means and methods reducing or preventing the generation of HDEP. To this purpose, novel packaging cells and methods of making these are provided.