Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins

摘要

The invention provides a method for producing at least one polypeptide of interest in a eukaryotic cell, the method comprising: providing a eukaryotic cell comprising nucleic acid encoding the polypeptide of interest; culturing the cell in a suitable medium; and harvesting the at least one polypeptide of interest from the eukaryotic cell, from the suitable medium, or from both the eukaryotic cell and the suitable medium, wherein the eukaryotic cell is a permanent amniocytic cell comprising a nucleic acid sequence encoding adenoviral E1A and E1B proteins. The invention also provides a eukaryotic cell comprising nucleic acid encoding a polypeptide of interest under control of a heterologous promoter, the eukaryotic cell not comprising a structural adenoviral protein, wherein the eukaryotic cell is a permanent amniotic cell comprising a nucleic acid sequence encoding adenoviral E1A and E1B proteins.