A NOVEL FOOT AND MOUTH DISEASE VIRUS ASIA 1 (INDIAN VACCINE STRAIN) REPLICON BASED VIRAL VECTOR FOR VACCINE RESEARCH AND DEVELOPMENT.

摘要

The invention deals with the development of a novel plasmid comprising replication competent cDNA copy of a Foot and mouth disease virus serotype Asia 1 (Indian Vaccine Strain Asia 1 63/72). This acts as a vector for easy selection of vaccine strains by inserting structural protein genes of identified field isolate to be developed as vaccine virus. For easy insertion of capsid protein genes a restriction site (Bam H1) was introduced into the vector. The vector can be used for development of tailor made vaccines which include insertion of desired marker epitopes,. development of dual vaccines or vaccines for human diseases. Functionality of the vector was tested using GFP epitope and structural protein genes of serotype'O'. The vector may be used for the development of DISC viruses or genetically modified attenuated FMDV or in the molecular studies for understanding the role of various proteins of the virus.