Method for marking of mitocu00d4ndria and retu00cdculo endoplasmu00c1tico using fluorescent compounds macrocu00cdclicos

摘要

This invenu00c7u00c3o relates to the use of the compounds macrocu00cdclicos me2 (28) py2n6, (16) phenn2, [32] phen2n4, (30) phen2n6 and 7,16,25,34 - di - N - hexil - (36) phen4n4 as specific probes of mitocu00d4ndrias and retu00cdculo endoplasmu00c1tico.This innovative method stands out for the ability of these compounds translocarem rapidly the membrane plasmu00c1tica of cells without prior conditioning of the same, not the damaging, exhibiting similar fluorescu00cancia intensities over 30 min and localized preferentially in the mitocu00d4n Drias and partially in the retu00cdculo endoplasmu00c1tico, maintaining the integrity of these organelles.It was demonstrated that these compounds are not citotu00d3xicos and do not induce apoptosis or necrosis in three kinds of cells studied (MCF - 7, NHDF and P14).The advantage of the use of these compounds as markers of these organelles is the low cost of organic synthesis and associated speed in obtaining the income razou00c1veis the inflammatory cycle - condensau00c7u00c3o, compounds having a positive charge and co locate quickly relocated PE Ru00cdodo below 30 min to mitocu00d4ndria and partially the retu00cdculo endoplasmu00c1tico.