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Effects of marking methods and fluorescent dusts on Aedes aegypti survival

机译:标记方法和荧光粉尘对AEDYPTI生存的影响

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Background Tracking the movement of mosquitoes and understanding dispersal dynamics is essential for the control and prevention of vector-borne diseases. A variety of marking techniques have been used, including dusts and dyes. Methods In this study, Aedes aegypti were marked using fluorescent dusts (‘DayGlo’: A-19 Horizon Blue & A-13-N Rocket Red; ‘Brian Clegg’: pink, blue & red), fluorescent paints (‘Brian Clegg’: blue, red & yellow) and metallic gold dust (‘Brian Clegg’). Dusting methods were those previously used in mark-release-recapture experiments, including application with a bulb duster, creation of a dust storm or shaking in a bag. Results Results showed marking mosquitoes using a dust storm allowed relatively high survival, compared to unmarked controls (Males: χ2?=?3.24, df?=?4, p?=?0.07; Females: χ2?=?3.24, df?=?4, p?=?0.04), and high marking efficiency. Using a bulb duster showed high survival in male mosquitoes (χ2?=?12.59, df?=?4, p?
机译:背景技术跟踪蚊子的运动和理解分散动态对载体疾病的控制和预防至关重要。已经使用了各种标记技术,包括灰尘和染料。方法在本研究中,AEDES AEGYPTI使用荧光粉尘('Dayglo':A-19 Horizo​​ n Blue&A-13-N Redred;'Brian Cleg':粉红色,蓝色和红色),荧光涂料('Brian Clegg' :蓝色,红色&黄色)和金属金灰尘('Brian Clegg')。除尘方法是先前用于标记释放的试验的那些,包括用灯泡除尘器的应用,在袋中产生尘埃风暴或摇动。结果结果表明,与未标记的对照相比,使用尘埃风暴的标记蚊子允许相对高的存活(男性:χ2?=?3.24,DF?=?4,P?= 0.07;女性:χ2?= 3.24,DF?= ?4,p?=?0.04),和高标记效率。使用灯泡喷粉器在雄性蚊子中显示出高的生存(χ2?= 12.59,df?=?4,p?<0.000),但在研究的前15天期间女性蚊子的生存率低(χ2?=?5.17 ,df?=?4,p?<?0.05)。与其他干标技术相比,灯泡除尘效率也具有最低的标记效率。在研究的前15天内,袋子方法在雄性中显示出低生存(χ2?=Δ5.77,DF?4,P?<0.05)。应用涂料对男性的生存有整体负面影响(χ2?=?5.03,DF?3,P?<0.05),但不是女性(χ2?=?0.19,DF?=?3,P? =?0.661)。雄性粉碎了Dayglo Horizo​​ n蓝色灰尘,而女性与Dayglo Rocket红色灰尘拂尘,与对照相比女性:χ2?=?24.47,df?=?6,p?<0.000)。标有Brian Clegg Gold Dust的蚊子表明了男性蚊子内的控制相似的死亡率(χ2?= 0.18,DF?=?6,P?=?0.674),但在女性中显着降低(χ2?=?16.59,DF? =?6,p?<?0.000)。结论本研究表明,标记技术和颜色可能对蚊子的生存和标记覆盖产生重大影响。

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