The present invention relates to a new technology for in vitro obtaining potato micro-tubers for seeding purposes, from healthy potato sprouts inoculated on a nutritive growing and rooting medium, MS 1962, wherefrom, in the growing chamber, after 3-4 weeks there are obtained plantlets of 5-6 cm which are subjected to micro-cutting. Each micro-cutting is then inoculated in medium MS 1962 additivated with α naphtyl acetic acid and solidified with agar-agar. After 3-4 weeks, from the micro-cuttings there is formed a new plantlet of 5-7 cm. The plantlet is cut in mini-cuttings, the operation being repeated as many times as necessary, with a 4-5 times multiplication at every 3-4 weeks. The resulting mini-cuttings are used for producing micro-tubers by their cultivation on a solid basic medium MS 1962 specific to the growing and rooting stage. The resulting 4-5 cm plantlets are, then, horizontally inoculated, 4-5/box, in the same medium. After 3-4 weeks, to the new plantlets, developed from each internode, there is added a tuberization medium comprising kinetin, coumarin and saccharose in a concentration of 80...90 g/l. After 6-8 weeks, the micro-tubers are harvested, there resulting the highest quantity of micro-tubers 10 mm and 1 g.
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