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A TRANSGENIC ANIMAL COMPRISING A DELETION OR FUNCTIONAL DELETION OF THE 3'UTR OF AN ENDOGENOUS GENE.
A TRANSGENIC ANIMAL COMPRISING A DELETION OR FUNCTIONAL DELETION OF THE 3'UTR OF AN ENDOGENOUS GENE.
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机译:内源性基因的3'UTR缺失或功能缺失的转基因动物。
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摘要
The present invention relates to the fields of knockout (KO) animal production. The invention is directed to a transgenic KO animal comprising a heterozygous or homozygous deletion or functional deletion of the gene's native 3' untranslated region (3'UTR) at least in one of its endogenous gene loci, wherein the disrupted endogenous gene is transcribed into an m RNA without its native 3'UTR. Instead, a 3'UTR of choice, knocked in by the experimenter, is transcribed into an m RNA. The 3'UTR KO animals provide a new approach to study gene function as they enable to overexpress the gene products what are negatively regulated via their 3'UTR-s exclusively in those cells that already transcribe the gene, thereby avoiding the misexpression problem present in the animals produced by conventional transgenesis methods. The invention is further directed to KO animals, in which the gene with deletion of 3'UTR is GDNF, NGF or BDNF.
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