A TRANSGENIC ANIMAL COMPRISING A DELETION OR FUNCTIONAL DELETION OF THE 3'UTR OF AN ENDOGENOUS GENE.

摘要

The present invention relates to the fields of knockout (KO) animal production. The invention is directed to a transgenic KO animal comprising a heterozygous or homozygous deletion or functional deletion of the gene's native 3 untranslated region (3UTR) at least in one of its endogenous gene loci, wherein the disrupted endogenous gene is transcribed into an m RNA without its native 3UTR. Instead, a 3UTR of choice, knocked in by the experimenter, is transcribed into an m RNA. The 3UTR KO animals provide a new approach to study gene function as they enable to overexpress the gene products what are negatively regulated via their 3UTR-s exclusively in those cells that already transcribe the gene, thereby avoiding the misexpression problem present in the animals produced by conventional transgenesis methods. The invention is further directed to KO animals, in which the gene with deletion of 3UTR is GDNF, NGF or BDNF.