METHOD FOR PREPARING AND RENATURING CHICKEN INTERFERON Γ

摘要

Provided is a method for preparing and renaturing chicken interferon γ, comprising the following steps: (1) performing PCR amplification on the genes of chicken interferon γ; (2) subcloning the product of step (1) to a pET32a expression vector having a 6-His tag; (3) transforming the product of step (2) into cells of Escherichia coli M15; (4) performing induction and expression on the product of step (3), then cracking to acquire a protein expression product; (5) purifying the expression product of step (4); and (6) renaturing recombinant protein purified to acquire a product. Employment of the pET32a and of Escherichia coli M15 allows for the expression product of chicken interferon γ gene to reach after purification a purity of 95%, which is greater than the 85% purity of a conventional method, and for the renaturation to reach a success rate between 38% and 39%, which is greater than the 20% success rate of the conventional method. Increase of active ingredient in total protein prepared improves treatment effects and reduces costs.