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Composition and methods for modifying gene expression using the promoter of ubiquitin conjugating protein coding gene of soybean plants

机译:利用大豆植物遍在蛋白缀合蛋白编码基因的启动子修饰基因表达的组合物和方法

摘要

The present invention comprises the description of a novel regulatory sequence provided to improve the expression of a nucleotide sequence, such as structural genes, in plants, including monocots and dicots. More specifically, the invention refers to regulatory sequences of polynucleotides isolated from soybean plants capable of initiating and activating the transcription of polynucleotides, and to the use of these regulatory sequences for modifying the transcription of endogenous and/or heterologous polynucleotides and the production of polypeptides. The invention also describes DNA constructs that contain the promoter of ubiquitin-conjugation protein coding gene of soybean plants operably linked to a heterologous and/or endogenous gene. Furthermore, the invention provides the use of these constructs in the form of expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs containing the promoter of ubiquitin conjugating protein coding gene of soybean plants for the production of plants, plant cells or transgenic protoplasts.
机译:本发明包括对新的调控序列的描述,该调控序列被提供用于改善植物(包括单子叶植物和双子叶植物)中核苷酸序列例如结构基因的表达。更具体地,本发明涉及从大豆植物中分离的能够启动和激活多核苷酸转录的多核苷酸的调控序列,并且涉及这些调控序列在修饰内源和/或异源多核苷酸的转录以及多肽生产中的用途。本发明还描述了DNA构建体,其包含与异源和/或内源基因可操作地连接的大豆植物的遍在蛋白缀合蛋白编码基因的启动子。此外,本发明提供了这些构建体以表达载体,重组载体的形式以及在植物,植物细胞或转基因原生质体中的用途。本发明还描述了一种使用这样的构建体的方法,该构建体包含大豆植物的遍在蛋白缀合蛋白编码基因的启动子,用于生产植物,植物细胞或转基因原生质体。

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