EPR METHOD FOR FREE RADICALS DETECTION USING AT LEAST TWO DIFFERENT SPIN TRAPPING COMPOUNDS

摘要

The present invention provides a method, a kit and their use for free radicals detection and identification in a sample. The method comprising the steps of: reacting said sample with a spin trapping composition, to produce spin adducts of said spin trapping composition and free radicals and detecting said spin adducts by electron paramagnetic resonance (EPR) spectroscopy or electron spin resonance (ESR), thereby obtaining an EPR signal. The method is characterized in that said spin trapping composition comprises at least two different spin trapping compounds.