PRODUCTION METHOD FOR PREPARING HIGH PURITY MANNAN OLIGOSACCHARIDE FROM ENZYMOLYSIS OF HEMICELLULOSE

摘要

A method for preparing a high purity mannan oligosaccharide from enzymolysis of a hemicellulose having a mannan oligosaccharide, also a product. By using a high-efficiency β-mannanase, BM-II, produced from fermentation of Bacillus subtilis strain TQBm having the deposit number of CCTCC No. M211147, Amorphophallus konjac gum, guar gum, locust bean gum, and Sesbania gum are hydrolyzed into relatively small molecules, then are separated by centrifugation, clarified by flocculation, desalinated, decolorized, and deodorized by using an ion exchange technology, then large molecules in a sugar solution are separated by using combinedly a super filtration technology and a molecular sieve chromatography technology, and finally the high purity mannan oligosaccharide is prepared via concentration and drying. The high purity mannan oligosaccharide acquired via the preparation is of low costs, free of chemical contamination, allows for implementation of industrialized production, significantly increases beneficial bacteria such as human intestinal bifidobacteria, significantly reduces harmful bacteria such as Enterobacter, also provides the effects of reductions in blood sugar and in blood lipids and of immunomodulation, and is a raw material and additive of a medicament for gastrointestinal tract health, for sugar reduction and lipids reduction, for immunomodulation, and for disease treatment and disease prevention.