METHOD FOR DETERMINING NUCLEOTIDE SEQUENCE OF DISEASE-RELATED NUCLEIC ACID MOLECULE IN SAMPLE TO BE TESTED

摘要

Disclosed is a method for determining the nucleotide sequence of a disease-related molecule in a sample to be tested, comprising: adding adaptors to the termini of DNA double-stranded nucleic acid fragments in the sample to be tested, the fragments being fragmented and derived from the genome, and enriched; the DNA double-stranded nucleic acid fragments containing the adaptors are captured using a nucleic acid chip, and the captured fragments are sequenced on a high-throughput sequencing platform. Also provided in the present invention are a nucleic acid chip able to be used in the abovementioned method and able to have from several to thousands of disease-specific probes immobilized thereon, and a kit containing the chip.