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Mutants of staphylokinase carrying amino and carboxy-terminal extensions for polyethylene glycol conjugation
Mutants of staphylokinase carrying amino and carboxy-terminal extensions for polyethylene glycol conjugation
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机译:带有氨基和羧基末端延伸的葡萄激酶激酶突变体,用于聚乙二醇偶联
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摘要
The present invention relates to the development of new derivatives of a bacterial plasminogen activator, Staphylokinase (SAK), having one or more amino acid residues with single or multiple cysteines at the amino and/or carboxy terminal ends and their conjugation with PEG (Polyethylene Glycol), resulting in new Staphylokinase derivatives that display altered oligomeric states, enhanced thermal and protease stability and extended plasma half-life. Also included is the cloning and expression in a suitable bacterial host; purification of Staphylokinase derivatives to homogeneity and their chemical modification by integrating a PEG molecule to create new biologically active Staphylokinases having higher protein stability and improved in vivo plasma half life, that may enhance the clinical potential of Staphylokinase in thrombolytic therapy for the treatment of cardiovascular diseases.
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