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Methods for obtaining thermostable enzymes, DNA polymerase I variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
Methods for obtaining thermostable enzymes, DNA polymerase I variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
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机译:从具有新催化活性的水生栖热菌中获得热稳定酶,DNA聚合酶I变体的方法,获得该酶的方法及其应用
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摘要
The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
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