SCAR MARKERS FOR DISCRIMINATION OF GRAPE CULTIVARS AND USE THEREOF

摘要

PURPOSE: A sequence characterized amplified region (SCAR) marker for determining the variety of grapes is provided to definitely distinguish the variety of grapes, to strengthen protection of plant variety right holders, and to be used in a research of molecular genetics and morphological characteristics of the variety. CONSTITUTION: A SCAR marker for determining the variety of grapes contains one or more selected from the group consisting of G509-243 (sequence number 35), GG05_281 (sequence number 36), GU19_283 (sequence number 37), G116_319 (sequence number 38), G507_334 (sequence number 39), G427_348 (sequence number 40), GU13_380 (sequence number 41), G427_382 (sequence number 42), G119_411 (sequence number 43), GV01_424 (sequence number 44), GW04_463 (sequence number 45), G169_515 (sequence number 46), G116_539 (sequence number 47), GV04_618 (sequence number 48), GV01_678 (sequence number 49), GG05_689 (sequence number 50), and GA07_775 (sequence number 51). The SCAR marker is acquired by random amplified polymorphic DNA (RAPD) analysis. A method for determining the variety of grapes comprises the steps of: isolating genome DNA from a grape; amplifying a target sequence by amplification using an oligonucleotide SCAR primer set and the genome DNA as a template; and detecting the amplified product.