SCREENING METHOD USING MG53-UBE2H INTERACTION FOR THE TREATMENT OF TYPE 2 DIABETES

摘要

PURPOSE: A screening method of a treatment medicine for type 2 diabetes using interaction of MG53 and UBE2H is provided to be able to effectively select and develop a treatment medicine for type 2 diabetes by screening materials inhibiting the interaction of UBE2H and MG53. CONSTITUTION: A screening method of a treatment medicine for type 2 diabetes comprises (a) a step of treating a cell expressing mitsugumin 53 (MG53) and ubiquitin-conjugating enzyme E2H (UBE2H) with a candidate compound; and (b) a step of determining whether or not the interaction of MG53 and UBE2H is inhibited in the cell. The interaction of MG53 and UBE2H is detected by using an antibody specific to MG53 and UBE2H. The interaction of MG53 and UBE2H is detected with the immunoblotting, immune precipitation or immunostaining.