首页> 外国专利> USE OF 2,4-DIACETYLPHLOROGLUCINOL HYDROLASE GENE AS A MARKER FOR SELECTING TRANSGENIC PLANTS

USE OF 2,4-DIACETYLPHLOROGLUCINOL HYDROLASE GENE AS A MARKER FOR SELECTING TRANSGENIC PLANTS

机译:2,4-二乙酰基甘油葡萄糖醇水解酶基因作为转基因植物选择的标志

摘要

PURPOSE: A system for sorting transgenic plants using a gene marker is provided to shorten sorting time with less contamination because seeds of a non-transformant cannot be germinated in a 2,4-diacetylphloroglucinol (DAPG) medium and to enable easy handling.;CONSTITUTION: A marker for sorting transgenic plants contains DAPG hydrolase gene (phlG) with a base sequence of sequence number 1. A recombinant plant expression vector contains the marker gene. A transgenic plant is prepared by transformation with a vector. A method for sorting transgenic plants comprises the steps of: transforming plant cells with the vector; and culturing the transformed plant cells in a DAPG-containing medium.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) 14 days later after sowing; (BB) 9 days later after sowing
机译:目的:提供一种使用基因标记对转基因植物进行分类的系统,以缩短分类时间,减少污染,因为非转化体的种子无法在2,4-二乙酰基间苯三酚(DAPG)培养基中发芽,并且易于处理。 :用于分选转基因植物的标记包含DAPG水解酶基因(phlG),其碱基序列为1号。重组植物表达载体包含该标记基因。通过用载体转化来制备转基因植物。一种用于分类转基因植物的方法,包括以下步骤:用载体转化植物细胞;和COPYRIGHT KIPO 2013; [参考数字](AA)播种后14天;然后在含有DAPG的培养基中培养转化的植物细胞。 (BB)播种后9天

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