MEDIUM FOR CULTURING HUMAN-DERIVED MONOCYTES AND A COMPOSITIOIN CONTAINING THE MONOCYTES AND ENDOTHELIAL PRECURSOR CELLS DIFFERENTIATED FROM THE MONOCYTES FOR TREATING ISCHEMIC DISEASES

摘要

PURPOSE: A medium for culturing monocytes is provided to improve survival ability and angiogenesis ability and to treat ischemia.;CONSTITUTION: A medium for culturing monocytes contains 20-400 ng/ml of each of EGF(epidermal growth factor), TPO(thrombopoietin), Flt-3L, IGF(insulin-like growth factor)-1, and bFGF(basic fibroblast growth factor) with a basal medium. The basal medium contains IMDM(Iscove`s Modified Dulbecco`s Medium) medium, DMEM, MEM, alpha-MEM, M-199, eagle`s basal medium, CMRL medium, RPMI 1640 medium, Ham`s F-10/12 medium, Fisher`s medium, McCoy`s 5A medium, Leibovitz`s medium, or MCDB. The medium additionally contains glutamine, FBS, penicillin, or streptomycin.;COPYRIGHT KIPO 2013