Methods for Screening TLR4(Toll-like receptor 4) Signaling Inhibitors and Uses Thereof

摘要

The invention is a step for containing the TLR4 (Toll-like receptor 4) signaling inhibitor screening to how the service is: (a) TLR4 (Toll-like receptor 4) or TLR4 extracellular domain on the test material in contact to step; And (b) in the case of a covalent bond to a cysteine ​​residue in the extracellular domain of comprising a step of analyzing whether or not the covalently attached to a cysteine ​​residue in the extracellular domain of the test substance TLR4, the test substances are TLR4 by inhibiting TLR4 oligomerization it is determined by TLR4 signaling inhibitors. The TLR4 signaling inhibitors, more preferably the present invention is a thiol-reactive compound (e.g., SFN) is shared with the cysteine ​​residues present in the TLR4 or TLR4 extracellular domain binding control form a duct (adducts), and a ligand of TLR4-induced to suppress all non-dependent oligomerization - and the oligomerization of TLR4 ligand. Thus, (e.g., SFN) TLR4 signaling inhibitor or an efficacy enhancer of the present invention, compositions comprising as an active ingredient was significantly attenuated the inflammatory response is effective in the treatment of inflammatory diseases the reduction of improved and swelling of the skin, even effective. ;