Probe composition for selectively enhancing signals of detecting target gene, probe composition for decreasing background signals, method for designing the probes and method for dectecting the target gene using the same

摘要

The present invention uses a labeled additional probe that specifically binds to a second specific region located adjacent to the first specific region in addition to the target probe that specifically binds to a first specific region of the target gene. It is possible to selectively enhance the detection signal and to easily set a cutoff value for determining the presence of the target gene, and to determine a first specific region of the target gene and a second specific region which is partially overlapped with the second target region of the target gene. Selecting a nonspecific background signal by nonspecific reaction with an allele of a target gene using an additional unlabeled probe that complementarily binds to a second specific region of the corresponding allele but does not bind specifically to a specific region Can be reduced. Therefore, the present invention provides a method for appropriately positioning a probe to be designed for a target gene, appropriate application criteria of a labeled additional probe for selective enhancement of a detection signal of a positive amplification product for a target gene, and a background signal due to a nonspecific reaction. It is possible to provide a method for reducing non-specific background signal by using an unlabeled additional probe for selective reduction of biomarkers, and to provide a clear method for determining monobasic polymorphism, and in bio, medical and pharmaceutical fields requiring analysis of genotyping or genotyping. It can be used in various fields such as.