Label-free Electrochemical Aptamer Sensor Using Nanoparticles

摘要

The present invention provides a specific binding with the aptamer using aptamer (aptamer) is a single-stranded nucleic acids as a protein (analyte) to electrochemically aptamer biosensor for quantitative analysis, at the end of aptamer enzyme or fluorescent substance, a method using non-labeled nanoparticles (nanoparticle) having an electrochemically active element radiating with a cover sheet such as a Par relates to an aptamer of the biosensor. Aptamer sensor of the present invention, simple and without a sandwich immunoassay in the form of labeled aptamer sensor using two different aptamer that was otherwise not use the existing research on the two aptamer, which can be measured in a short time non- a sensor of the labeling method, by electrochemical synthesis of nanoparticles caught as soon as the electrochemically active To measure, build methodology by protein (analyte) for quantitative analysis (methodology) to a specific binding with the aptamer by using the was. Aptamer sensor of the present invention was significantly improving the problems of low accuracy and repeatability experiment comes from the existing complicated process.