IMMUNOLOGICAL RESEARCH USING A NON-DISPERSED CHEMILUMINESCENT REAGENT

摘要

1. A chemiluminescent reagent for determining the presence and / or amount of an analyte in a sample with an estimated analyte content, wherein the reagent is non-dispersed and contains a binding partner for the analyte and a chemiluminescent composition containing an olefin compound and a metal chelate. 2. The chemiluminescent reagent according to claim 1, wherein the binding partner for the analyte is covalently linked to the chemiluminescent composition via a bond or a linking group. The chemiluminescent reagent according to claim 2, wherein the binding partner for the analyte is covalently linked to the chemiluminescent composition via a linking group, and in which the linking group contains a hydrophilic macromolecule. The chemiluminescent reagent according to claim 3, wherein the olefin compound and the metal chelate are bonded to the same molecule of the binding group to which the binding partner for the analyte is covalently bonded. The chemiluminescent reagent according to claim 3, wherein the olefin compound and the metal chelate are connected to separate the molecules of the binding group to which the binding partner for the analyte is covalently linked, and in which the binding group may be the same or different. The chemiluminescent reagent according to claim 3, wherein the hydrophilic macromolecule is selected from the group consisting of polypeptides, dendrimers, polycarboxylates, polyamines, polysulfhydryls and polyethylene glycols. The chemiluminescent reagent according to claim 1, wherein the binding partner for the analyte is non-covalently bound to chemilumines