METHOD OF DETECTION OF THE STRAINS OF TUBERCULOSIS VERSATILE BY PLURAL PACK STABILITY WITH USE OF MASS SPECTROMETRY

摘要

1. A method for determining Mycobacterium tuberculosis strains of multidrug resistant using mass spectrometry based on checking using mass spectrometry in the presence of nucleotide substitutions loci rpoB, katG, fabG-inhA (promoter region) and embB, substitutions scanned position is given in Table 2 , the presence of at least one of the substitutions in the rpoB indicates the strain resistant to rifampicin, the presence of at least one of the substitutions in the katG or fabG-inhA evidence of strain resistance izoniazidu.2. A method according to claim 1, characterized in that the amplification of the loci is performed using primers, which sequences are shown in Table 1.3. A method according to claim 1, characterized in that the reaction is carried out in a thermocyclic minisequencing reaction mixture: 66 mM Tris-HCl pH 9,0; 16.6 mM (NH4) SO; 2.5 mM MgCl; 0.2 mM dNTPs necessary and / or a ddNTP; 10 pmoles of appropriate primer sequences are given in Table 2.