BASICALLY NON-TOXIC MUTEIN OF ALPHA-TOXIN Clostridium perfringens AND ITS NUCLEIC ACID CODING MOLECULE

摘要

1. A nucleic acid molecule encoding a substantially non-toxic mutein of the alpha toxin Clostridium perfringens; moreover, the alpha toxin mutein contains the amino acid sequence of SEQ ID NO: 3, with the exception of at least 9 consecutive amino acid residues; and wherein one of the deleted amino acid residues is His. 2. The nucleic acid molecule according to claim 1, wherein the alpha toxin mutein contains the amino acid sequence of SEQ ID NO: 3, with the exception of at least 12 consecutive amino acid residues; and wherein one of the deleted amino acid residues is His. 3. The nucleic acid molecule according to claim 2, wherein the alpha-toxin mutein contains the amino acid sequence of SEQ ID NO: 3, with the exception of at least 18 consecutive amino acid residues; and wherein one of the deleted amino acid residues is His. 4. The nucleic acid molecule according to claim 1, wherein the alpha-toxin mutein contains SEQ ID NO: 3, with the exception of 9 consecutive amino acid residues between Tyr and Trp. 5. The nucleic acid molecule according to claim 1, containing the nucleic acid sequence of SEQ ID NO: 2; wherein nucleotides 268-294 of the nucleic acid molecule are deleted. The nucleic acid molecule of claim 5, wherein the deleted nucleotides are replaced by a nucleotide sequence encoding a single Leu residue. Mostly non-toxic mutein of alpha-toxin Clostridium perfringens; moreover, the alpha toxin mutein contains the amino acid sequence of SEQ ID NO: 3, with the exception of at least 9 consecutive amino acid residues; and wherein one of the deleted amino acid residues is His.8. Mostly non-toxic mutein alpha toxin