METHOD FOR PRODUCING TEST SYSTEMS 'Khanty-of N' FOR THE DETERMINATION OF SPECIFIC ACTIVITY OF VACCINE hemorrhagic fever with renal syndrome

摘要

1. A process for preparing the immunoenzyme test system «XAHTA-N» for evaluation of immunospecific proteins Puumala hantaviruses, Dobrava, Hantaan virus and Seoul in vaccine formulations against hemorrhagic fever with renal syndrome (HFRS), characterized in that the virus-derived monoclonal Puumala and Dobrava antibodies selected according to the principle of cross specificity for antigens Puumala virus, Dobrava, Hantaan, Seoul, are adsorbed onto the surface of the polystyrene panels, and also used for the preparation of conjugate peroxidase hrena.2. The method of claim 1, wherein the immunoassay test system «XAHTA-N» identifies antigens all 4 hantaviruses - HFRS pathogens that allows to unify control methods in specific activity polyvalent vaccines against GLPS.3. A method according to claim 1, characterized in that the sensitivity of the same (according to virus titer) antigens defined by each of hantavirus 4 - GLPS.4 pathogens. A method according to claim 1, characterized in that with the same sensitivity as the detected antigens are live and formalin-inactivated virus, which is important from the point of view of the specific activity of inactivated vaccines on their production process steps.