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QUANTITATIVE FOOD PROTEINS CONTENT DETERMINATION METHOD

机译:食品中蛋白质含量的测定方法

摘要

FIELD: food industry.;SUBSTANCE: quantitative food proteins content determination method involves the following operations, sequentially performed: mixing test samples of the substrate and a fermentative substance coupled with a stabilising solution, incubation of the produced mixture at a temperature of 37°C, centrifugation of the fermentative-and-substrate complex and determination of quantitative food proteins content by way of calculation. The fermentative substance is represented by pancreatic juice preliminarily diluted with a stabilising solution down to 50% concentration, the ratio of the solution to the substrate test sample weight equal to 1:10. The prepared mixture incubation is performed during 5-15 minutes. Before quantitative food proteins content determination by way of calculation, the pure liquid fraction volume produced as a result of centrifugation is diluted with the stabilising solution at a ratio of 1:100-200. The food protein quantity is determined as equal to percentage expenditure of protease enzymes in comparison with a reference sample of pancreatic juice solution.;EFFECT: enhanced accuracy of determination of the quantitative food proteins content in food products.;3 tbl
机译:领域:食品工业;物质:定量食物蛋白质含量的测定方法涉及以下操作,依次进行:将底物的测试样品与发酵液与稳定液混合,将制得的混合物在37°C下温育,发酵和底物复合物的离心分离以及通过计算确定定量食物中蛋白质的含量。发酵物质以胰液为代表,该胰液预先用稳定溶液稀释至浓度为50%,该溶液与底物测试样品重量之比等于1:10。制备的混合物孵育时间为5-15分钟。在通过计算确定定量食物蛋白质含量之前,将通过离心产生的纯净液体部分体积用稳定溶液以1:100-200的比例稀释。确定食品蛋白质的量等于与胰液溶液参考样品相比蛋白酶的消耗百分比。效果:提高食品中定量食品蛋白质含量的确定准确性。3tbl

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