METHOD FOR DETECTING HANTAAN VIRUS ANTIBODIES IN HANTAAN HEMORRHAGIC FEVER, AND PREDICTING SEVERE DISEASE

摘要

FIELD: medicine.;SUBSTANCE: method involves the electrophoretic detection of the polypeptide composition of an antigenic preparation of Hantaan virus, the detection of the Hantaan virus antibodies in blood serum by immunoblot analysis with the IgG antibodies detected in circulating immune complexes (CICs) with the use of an antigenic preparation purified and concentrated by ultracentrifugation in a self-forming density gradient of potassium bromide. If observing the IgG antibodies to nucleocapsid protein with a molecular weight of 45 kDa in the slow precipitating circulating immune complexes, a severe form of Hantaan hemorrhagic fever is diagnosed at the early stages.;EFFECT: method enables higher effectiveness and specificity of detecting the Hantaan virus antigen antibodies in Hantaan hemorrhagic fever and specifying the predictors of the severe disease.;2 cl, 1 dwg, 1 tbl, 2 ex