PERMANENT OMG CELL LINE FROM GONADS OF TROUT (ONCORHYNCHUS MYKISS)

摘要

FIELD: biotechnologies.;SUBSTANCE: permanent line of cells is produced from tissue of trout gonads by means of long-term passage in the medium of Igla MEM on Earl salts with 25 mM of HEPES from 10% embryonic serum. The line may be used as a test culture for extraction, accumulation, titration and study of a infectious pancreas necrosis virus (IPNV) of salmon fishers, virulent haemorrhagic septicemia virus (VHSV) of salmon fishes, infectious hemopoietic necrosis virus (IHNV) of salmon fish tissue, spring viraemia of carps virus (SVCV), koi herpes virus (KHV), sturgeon herpes virus (SbSHV).;EFFECT: permanent line of OMG cells will find wide application in laboratory research on virosology and biotechnology in production of virus vaccines and diagnosticums.;1 tbl