METHOD OF LASER FUSION OF BLASTOMERES INSIDE THE EARLY PREIMPLANTATION MAMMALIAN EMBRYOS WITHOUT VIOLATION OF THEIR INTEGRITY

摘要

FIELD: biotechnology.SUBSTANCE: method of laser fusion of blastomeres inside the 4-cell mouse embryo without violation of its integrity, comprising demonstration of the embryo on the monitor and exposure with laser pulses of the visually selected point on the line of close contact of blastomeres inside the embryo. Either two blastomeres inside the 4-cell embryo are subjected to fusion to form the embryo of three cells one of which is tetraploid, or two pairs of blastomeres in series to form the embryo of two tetraploid cells, or three blastomeres in series to form the embryo of two cells of different size: hexaploid and intact diploid. Irradiation is carried out with the sequence of femtosecond laser pulses with a wavelength of 800 nm with a repetition rate of 80 MHz with the wattage of 0.06-0.12 W and the sequence duration of 10-30 ms. To find the area of the most close contact of the blastomeres inside the embryo the laser tweezers is used, enabling to move and rotate the embryo on the monitor.EFFECT: method with the influence of femtosecond laser irradiation enables to carry out a successful fusion of blastomeres inside the 4-cell mouse embryo without violation of its integrity to obtain embryos different in structures, containing cells of different ploidy.2 cl, 20 dwg