Supplementation of kinetin for mammalian preimplantation embryo development

摘要

A method for culturing an embryo in early mammal pregnancy using kinetin is provided to increase the rate of expanded and hatched blastocysts, growth rate and survivability of the blastocysts. A method for developing an embryo in early mammal pregnancy using NCSU(North Carolina State University-23 medium)-PVA(polyvinylalcohol) comprises a step of culturing the embryo in a culture solution of NCSU(North Carolina State University-23 medium)-PVA(polyvinylalcohol) where 100-800muM/L of kinetin is added at a temperature of 37-39 deg.C for 5-7 days.