A hypoxic pressure chamber or incubator for cell culture comprises an air tight plastic box that has: at least one gas inlet; and a gas outlet, the gas outlet being closed with an adjustable one way valve (i.e. a check or pressure relief valve) that is configured to allow a desired increased pressure level inside the box (preferably up to 20 cm H2O, 49.82 mbar). Preferably, a medical positive end expiratory pressure valve (PEEP valve) is conveniently utilised as the one way valve and the incubator preferably portable. In some embodiments the incubator may comprise a more than one gas inlet, i.e. a tri-gas hypoxic incubator to allow culture gas mixing.
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机译:用于细胞培养的低氧压力室或培养箱包括一个气密的塑料盒,该塑料盒具有:至少一个气体入口;和气体出口,该气体出口由可调节的单向阀(即单向阀或泄压阀)关闭,该单向阀被配置为允许在箱内增加所需的压力水平(最好达到20 cm H2O,49.82 mbar)。优选地,医用正向呼气压力阀(PEEP阀)被方便地用作单向阀,并且培养箱优选地是便携式的。在一些实施方案中,培养箱可包括一个以上的气体入口,即三气体低氧培养箱,以允许培养气体混合。
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