Two-step nucleic acid testing method using the same sample

摘要

The present invention provides a method of testing whether a sequence of interest is contained in a sample nucleic acid or not, comprising performing a simple test on the sample nucleic acid and subjecting the same sample nucleic acid to a close examination when it has been judged that the sequence of interest is contained therein or when it has been judged that the sequence of interest is not contained therein; and a method of testing whether a sequence of interest is contained in a sample nucleic acid in more than a specific amount or not, comprising performing a simple quantitative test on the sample nucleic acid and subjecting the same sample nucleic acid to a close examination when it has been judged that the sequence of interest is contained therein in more than the specific amount. According to these methods, it becomes possible to examine individuals, species, habitats, etc. of organisms with much higher reliability than that in conventional techniques.