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Parthenogenetic activation of human oocytes to produce human embryonic stem cells
Parthenogenetic activation of human oocytes to produce human embryonic stem cells
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机译:人卵母细胞的孤雌生殖活化产生人胚胎干细胞
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摘要
Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O2 tension, including manipulation of Ca2+ under high O2 tension and contacting oocytes with serine threonine kinase inhibitors under low O2 tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high O2 tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing ICM/stem cells. Stem cells produced by the disclosed methods are also described.
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