Surface plasmon excitation fluorescence measurement method and surface plasmon excitation fluorescence measuring device

摘要

To provide a surface plasmon excitation fluorescence measurement method and surface plasmon excitation fluorescence measuring device to improve the accuracy and sensitivity of the detection of the antigen. Fluorescent labeled antibody is allowed to bind to antigen the antigen captured by the antigen capture assay chip body, captured in the antigen capture body. light intensity of the surface plasmon excited fluorescence emitted from the fluorescent label when the excitation light is incident on the analysis chip is measured. Resonance angle θr is measured before the fluorescent labeled antibody is allowed to bind to the antigen even after the antigen was captured by the antigen capture body. Measurement angle θm is determined from the resonance angle θr. The incident angle θ of the excitation light to the reflective surface is set in resonance angle θr, light intensity of the surface plasmon excited fluorescence is measured.