Gene identification method and expression analysis method in exhaustive fragment analysis

摘要

A database construction method, including a stage that fragments genomic DNA contained in a sample or cDNA obtained from a transcription product and obtains a fragment DNA mixture by applying an identifiable index array, a stage that performs high-speed DNA sequencing of a portion of the fragment DNA mixture and acquires read array data for all fragment DNA contained therein, a stage that detects the presence of the index array portion for all read array data and extracts the read array data having the index array, and a stage that performs clustering and assembling of the sequences using sequence similarity and sequence length parameters, forms a plurality of clusters, and acquires the number of structural sequences of the cluster, consensus sequence and consensus sequence length, and alignment information for the clusters.