Identification of gene profiles of CD4~+ and CD8~+ T lymphocyte in systemic lupus erythematosus by generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification

摘要

Objective To identify LongSAGE Tags in systemic lupus erythematosus (SLE) by generation of longer cDNA fragments from serial analysis of gene expression (SAGE) tags for gene identification (GLGI). Methods CD4+ and CD8+ T lymphocytes were collected from the PBMCs of 25 patients with SLE and 10 healthy controls. Then the total RNA was extracted and reversely