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Method for producing soluble feather keratin protein

机译:可溶性羽毛角蛋白的制备方法

摘要

PROBLEM TO BE SOLVED: To easily convert a feather, that is produced in treatment of waste chicken or broiler and difficult to effectively use as it is, into soluble feather keratin protein.;SOLUTION: Feather for bedding inner pad is washed with a neutral detergent, washed with water and dried. The feather in an amount of 1 g is fed to a flask of 300 mL, mixed with 50 mL of a thioglycolic acid solution having 0.2 mol/L concentration adjusted to pH 11.0 with sodium hydroxide, stirred and shaken at 30°C for 48 hours, the solution is adjusted to 30°C and adjusted to pH 7 with acetic acid. The solution is mixed with 50 mL of sodium bromate solution having 0.4 mol/L concentration by gradually adding the sodium bromate solution over 4 hours, then the solution is allowed to stand for 20 hours. Then insoluble matter is filtered off from the solution, the solution is adjusted to approximately pH 3.5 with acetic acid, a polymer substance is precipitated and the precipitate is recovered by centrifugal separation. The precipitate is made into a paste state acidic with acetic acid and preserved at a dark cold place. The solubilization does not cause the reduction in molecular weight.;COPYRIGHT: (C)2010,JPO&INPIT
机译:解决的问题:要轻松地将处理废鸡或肉鸡时产生的,难以按原样有效使用的羽毛转化为可溶性羽毛角蛋白。;解决方案:用于床上用品内胆的羽毛要用中性洗涤剂清洗,用水洗涤并干燥。将1g的羽毛喂入300mL的烧瓶中,与50mL的具有0.2mol / L浓度的硫代乙醇酸溶液用氢氧化钠混合,将其调节至pH 11.0,在30℃下搅拌并摇动48小时。然后,将溶液调节至30℃并用乙酸调节至pH 7。通过在4小时内逐渐添加溴酸钠溶液,将溶液与50mL浓度为0.4mol / L的溴酸钠溶液混合,然后将溶液静置20小时。然后从溶液中滤出不溶物,用乙酸将溶液的pH值调节至约3.5,使聚合物质沉淀,并通过离心分离回收沉淀物。将沉淀物用乙酸制成酸性的糊状,并保存在暗处。增溶作用不会引起分子量的降低。;版权所有:(C)2010,JPO&INPIT

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